Production Optimization of Extracellular L-asparaginase through Solid- State Fermentation by Isolated Bacillus Subtilis
نویسنده
چکیده
Susmita Shukla and S.K. Mandal Department of Biochemical Engineering and Food Technology, Harcourt Butler Technological Institute, Kanpur-208002, India. E-mail: [email protected] ABSTRACT: L-asparaginase has been used as anti-tumor agent for the treatment of acute lymphoblastic leukemia and food processing aid to reduce the formation of cancer causing acrylamide. Extracellular Lasparaginase production was optimized through solid state fermentation using ground nut cake by isolated Bacillus subtilis. which was not reported in literature.Optimum production of L-asparaginase enzyme (18.4U/ml) was obtained after 48h of incubation at 37C moisture content of 70% and at pH 7.
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Production Purification and Characterization of Extracellular Anti-leukaemic L-asparaginase from Isolated Bacillus Subtilis Using Solid State Fermentation
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